Again, pharma/biopharma was a hot topic at the 42nd ISCC in Riva del Garda. Using data elaborated by Christof Regl and Therese Wohlschlager, Christian Doppler Laboratory Head Christian Huber presented a critical check on the capabilities of chromatography and mass spectrometry with respect to revealing oxidation in biopharmaceuticals. Details will follow soon in a short article to be published in the LCGC Biopharmacuetical Perspectives Column.
After a number of struggles, Therese Wohlschlager's work on deciphering highly complex glycosylation in a therapeutic fusion protein using enzymatic tools and native mass spectrometry finally got published in Nature Communications. Full text is available here.
Wolfgang Skala's manuscript entitled "MoFi – A software tool for annotating glycoprotein mass spectra by integrating hybrid data from the intact protein and glycopeptide level" was on-line published in Analytical Chemistry - congratulations! The preprint is available here.
Therese Wohlschlager's findings on the plurality of protein isoforms of Etanercept triggered Neil Kelleher's (Northwestern University) interest and resulted in an invitation to contribute to a large collaborative perspective article in Nature Chemical Biology dealing with the essential question "how many proteoforms are there?"
Nature Chemical Biologyvolume 14, pages 206–214 (2018)
Julia Hollerweger's work on analytical cascade of enzymes (ACE) for detection of protein structural variations has been published in Analytical Chemistry. Congratulations! The preprint is available here.
P.I.s, PostDocs and PhD students of the bioanalytical labs of Salzburg, Kiel, and Strasbourg met for the first time in Strasbourg to discus and exchange methods of protein analytis and characterization mostly in the biopharma area.
Our host, Sarah Cianferani, with our guest star, Alain Beck, in the back, who kindly joined the meeting via video conference, giving a very interesting overview about curent status of biopharmaceutical developemnt and analytical challenges involved. The group decided to hold the next meeting in March 2018 in Salzurg.
Good ideas best grow on the basis of a nice and communicative dinner! Strasbourg is a great place to get inspired!
Luigi Grassi's, Christof Regl's and Mario Schubert's paper on the assignment of succinimide chemical shifts in peptides and proteins got accepted in Analytical Chemistry ( DOI: 10.1021/acs.analchem.7b01645).