Paper Publications

Peer-reviewed

Stock L. G., Wildner S., Regl C., Gadermaier G., Huber C. G., Stutz H.
Monitoring of Deamidation and Lanthionine Formation in Recombinant Mugwort Allergen by CZE-UV and tCITP-ESI-TOF-MS
Anal. Chem. 90(20), 11933–11940 (2018)

Wohlschlager T., Regl C., Huber C. G.
Monitoring of oxidation in biopharmaceuticals with top-to-bottom high performance liquid chromatography–mass spectrometry methodologies: A critical check
LCGC Europe 31(6), 326–331 (2018)

Skala W., Wohlschlager T., Senn S., Huber G. E., Huber C. G.
MoFi: A software tool for annotating glycoprotein mass spectra by integrating hybrid data from the intact protein and glycopeptide level
Anal. Chem. 90(9), 5728–5736 (2018)

Wohlschlager T., Scheffler K., Forstenlehner I. C., Skala W., Senn S., Damoc E., Holzmann J., Huber C. G.
Native mass spectrometry combined with enzymatic dissection unravels glycoform heterogeneity of biopharmaceuticals
Nat. Commun. 9(1), 1713 (2018)

Pablos I., Eichhorn S., Machado Y., Briza P., Neunkirchner A., Jahn-Schmid B., Wildner S., Soh W. T., Ebner C., Park J. W., Pickl W. F., Arora N., Vieths S., Ferreira F., Gadermaier G.
Distinct epitope structures of defensin-like proteins linked to proline-rich regions give rise to differences in their allergenic activity
Allergy 73(2), 431–441 (2018)

Roschger C., Schubert M., Regl C., Andosch A., Marquez A., Berger T., Huber C. G., Lütz-Meindl U., Cabrele C.
The recombinant inhibitor of DNA binding Id2 forms multimeric structures via the helix-loop-helix domain and the nuclear export signal
Int. J. Mol. Sci. 19(4), E1105 (2018)

Hollerweger J. C., Hoppe I. J., Regl C., Stock L. G., Huber C. G., Lohrig U., Stutz H., Brandstetter H.
Analytical Cascades of Enzymes for sensitive detection of structural variations in protein samples
Anal. Chem. 90(8), 5055–5065 (2018)

Aebersold R., Agar J. N., Amster I. J., Baker M. S., Bertozzi C. R., Boja E. S., Costello C. E., Cravatt B. F., Fenselau C., Garcia B. A., Ge Y., Gunawardena J., Hendrickson R. C., Hergenrother P. J., Huber C. G., Ivanov A. R., Jensen O. N., Jewett M. C., Kelleher N. L., Kiessling L. L., Krogan N. J., Larsen M. R., Loo J. A., Ogorzalek Loo R. R., Lundberg E., MacCoss M. J., Mallick P., Mootha V. K., Mrksich M., Muir T. W., Patrie S. M., Pesavento J. J., Pitteri S. J., Rodriguez H., Saghatelian A., Sandoval W., Schlüter H., Sechi S., Slavoff S. A., Smith L. M., Snyder M. P., Thomas P. M., Uhlén M., Van Eyk J. E., Vidal M., Walt D. R., White F. M., Williams E. R., Wohlschlager T., Wysocki V. H., Yates N. A., Young N. L., Zhang B.
How many human proteoforms are there?
Nat. Chem. Biol. 14(3), 206–214 (2018)

Zauner F. B., Dall E., Regl C., Grassi L., Huber C. G., Cabrele C., Brandstetter H.
Crystal structure of plant legumain reveals a unique two-chain state with pH-dependent activity regulation
Plant Cell 30(3), 686–699 (2018)

Participation in Conferences

Invited Talks

Huber C. G.
Characterization of post-translational modifications in therapeutic proteins by HPLC-MS and native MS
Thermo Scientific Biopharma Summit, Shaping the Future Together, Vienna (Austria), 01.03.2018

Gadermaier G.
Detection of proteins and biopharmaceuticals using aptamers
Analytica, München (Germany), 10.04.2018

Huber C. G.
HPLC-MS and Native MS Approaches to the Characterization of Posttranslational Modifications in Biopharmaceuticals
Analytica, München (Germany), 12.04.2018

Huber C. G.
Microscale and capillary scale HPLC-MS methods for biopharmaceutical analysis
International Symposium on Capillary Chromatography, Riva del Garda (Italy), 13.–18.05.2018

Huber C. G.
Protein glycosylation analysis employing mass spectrometry and bioinformatics: the holy grail in therapeutic protein analysis
EUPA Summer School on Practical Proteomics, Vienna (Austria), 16.–20.07.2018

Huber C. G.
Molecular Characterization of Biotherapeutic Proteins: Concepts and Challenges for Separation Science and Mass Spectrometry
47th International Symposium on High-Performance Liquid Phase Separations and Related Techniques (HPLC 2018), Washington (USA), 29.07.–02.08.2018

Huber C. G.
Physiko-chemische Charakterisierung therapeutischer Proteine mittels HPLC-MS
ASAC Chromatographie und Kopplung mit Massenspektrometrie, summer school, Seggau (Austria), 09.–12.09.2018

Wohlschlager T.
Native mass spectrometry unravels highly complex glycosylation patterns in biopharmaceuticals
10th ÖGMBT Annual Meeting, Vienna (Austria), 17.–20.09.2018

Huber C. G.
Molecular Characterization of Biotherapeutic Proteins: Concepts and Challenges for Separation Science and Mass Spectrometry
Fabian 2018 Symposium of the Dutch Workgroup of Pharmaceutical and Biomedical Analysis, Nijmegen (The Netherlands), 05.10.2018

Huber C. G.
In-depth characterization of therapeutic proteins by chromatography and mass spectrometry: challenges and potential
15th International Interdisciplinary Meeting on Bioanalysis (CECE 2018), Brno (Czech Republic), 15.–17.10.2018

Talks

Regl C.
A Generic HPLC Method for Absolute Quantification of Oxidation in Monoclonal Antibodies and Fc-Fusion Proteins Using UV and MS Detection
35th Agilent Forum Analytik, Vienna (Austria), 13.02.2018

Schubert M.
Detection of succinimide as degradation product in proteins
Central European NMR Meeting, Valtice (Czech Republic), 23.–25.05.2018

Wohlschlager T.
Native mass spectrometry unravels highly complex glycosylation patterns in biopharmaceuticals
Austrian Proteomics and Metabolomics Research Symposium (APMRS) 2018, Vienna (Austria), 29.–31.08.2018

Stutz H.
Characterization of proteins/allergens applying a CE-toolbox
ASAC Minisymposium: Perspectives of capillary electrophoresis, Vienna (Austria), 15.11.2018

Poster Presentations

Wohlschlager T.
Dilute-and-Shoot Analysis of Therapeutic Monoclonal Antibody Glycosylation from Fermentation Broth: A Method Capability Study
32nd International Symposium on Chromatography ISC 2018, Cannes-Mandelieu (France), 23.09.2018

Hinterholzer A.
The NMR fingerprint of pyroglutamate and its application in the characterization of intact proteins
8th Austrian Peptide Symposium, Salzburg (Austria), 13.12.2018

Participation

Hinterholzer A.
Central European NMR Meeting, Valtice (Czech Republic), 23.–25.05.2018

Hinterholzer A.
Bruker Central European User’s Meeting, Vienna (Austria), 05.–06.09.2018

Hinterholzer A., Schubert M.
NMR Symposium "The Future of Integrative Structural Biology", Munich (Germany), 19.10.2018

Scientific Awards and Honours

Wohlschlager T.
Publication of the Month May
Department of Biosciences, University of Salzburg, for her paper in Nature Communications (see above)

Wohlschlager T.
Best oral presentation award
APMRS 2018, 31.08.2018

Wohlschlager T.
ÖGMBT Life Science Research Award
17.09.2018

Wohlschlager T.
Shimadzu best poster award
ISC 2018, 27.09.2018

Stock L. G.
Publication of the Month November
Department of Biosciences, University of Salzburg, for his paper in Analytical Chemistry (see above)

Wohlschlager T.
Publication of the Year
Department of Biosciences, University of Salzburg, for her paper in Nature Communications (see above)

Organisation of Scientific Events

35th Winterschool on Proteinases and Inhibitors
Tiers (Italy), 28.02.–04.03.2018

1st SKS Meeting
Strasbourg (France), 09.03.2018

National and International Cooperations

A. Bathke, W. Trutschnig
Statistics and Probability Research Group, Department of Mathematics, University of Salzburg, Austria

J. Bones
National Institute for Bioprocessing Research & Training (NIBRT), Dublin, Ireland

O. Kohlbacher
Applied Bioinformatics Group, University of Tübingen, Germany

C. Herwig
CD-Laboratory for Mechanistic and Physiological Methods for more Efficient Bioprocesses, Technical University Vienna, Austria

N. Kelleher
Northwestern University, Chicago, IL (USA)

A. van Dorsselaer
Université de Strasbourg, France