Paper Publications

Peer-reviewed

Regl C., Wohlschlager T., Esser-Skala W., Wagner I., Samonig M., Holzmann J., Huber C. G.
Dilute-and-shoot analysis of therapeutic monoclonal antibody variants in fermentation broth: a method capability study.
MAbs 11(3), 569–582 (2019)

Wildner S., Huber S., Regl C., Huber C.G., Lohrig U., Gadermaier G.
Aptamers as quality control tool for production, storage and biosimilarity of the anti-CD20 biopharmaceutical rituximab
Sci. Rep. 9(1), 1111 (2019)

Schweida D., Barraud P., Regl C., Loughlin F. E., Huber C. G., Cabrele C., Schubert M.
The NMR signature of gluconoylation: a frequent N-terminal modification of isotope-labeled proteins
J. Biomol. NMR 73(1–2), 71–79 (2019)

Wildner S., Griessner I., Stemeseder T., Regl C., Soh W. T., Stock L. G., Völker T., Alessandri C., Huber C. G., Stutz H., Brandstetter H., Gadermaier, G.
Boiling down the cysteine-stabilized LTP fold - loss of structural and immunological integrity of allergenic Art v 3 and Pru p 3 as a consequence of irreversible lanthionine formation
Mol. Immunol. 116, 140–150 (2019)

Hinterholzer A., Stanojlovic V., Cabrele C., Schubert M.
Unambiguous identification of pyroglutamate in full-length biopharmaceutical monoclonal antibodies by NMR spectroscopy
Anal. Chem. 91(22), 14299–14305 (2019)

Grassi L., Cabrele C.
Susceptibility of protein therapeutics to spontaneous chemical modifications by oxidation, cyclization, and elimination reactions
Amino Acids 51(10–12), 1409–1431 (2019)

Without peer review

Gadermaier G., Kohlberger M., Wildner S.
Screening protein surface biosimilarity of rituximab using aptamers
Generic and Biosimilars Initiative (GaBI), online 2019-09-29

Participation in Conferences

Invited Talks

Huber C. G.
The search for impurities in low- and high molecular pharmaceuticals: two different worlds?
Anakon 2019, Münster (Germany), 25.–28.03.2019

Huber C. G.
Impurity profiling in conventional pharmaceuticals and biopharmaceuticals: HPLC-MS methodology at the limits?
48th International Symposium on High-Performance Liquid Phase Separations and Related Techniques (HPLC 2019), Milano (Italy), 16.–20.06.2019

Stutz H.
Alliances of different CE and CE-MS approaches for the PTM characterization of allergens (Keynote lecture)
25th International Symposium on Electroseparation and Liquid Phase Separation Techniques (ITP 2019), Toulouse (France), 01.–04.09.2019

Brandstetter H.
Translational Research at the Department of Biosciences
Academia-Industry Partnership in Salzburg, Salzburg (Austria), 07.10.2019

Huber C. G.
Molecular Attributes of Protein Biotherapeutics: Concepts and Challenges for Separation Science, Mass Spectrometry and Computational Biology
GDCH Kolloquium, University of Regensburg, Regensburg (Germany), 25.11.2019

Schubert M.
Identification of protein modifications by NMR spectroscopy
9th Austrian Peptide Symposium, University of Vienna, Vienna (Austria), 05.12.2019

Talks

Schubert M.
Gluconoylation - a frequent N-terminal modification - studied in isotope-labeled proteins by NMR spectroscopy
23rd Austrian Carbohydrate Workshop, Graz (Austria), 14.–15.02.2019

Wohlschlager T.
Native mass spectrometry unravels highly complex glycosylation patterns in the therapeutic Fc-fusion protein Etanercept
Celebration of Native Mass Spectrometry, Oxford (United Kingdom), 24.–26.03.2019

Kohlberger M.
Aptamers, folded single-stranded oligonucleotides, are able to detect heat induced epitopes of rituximab, a monoclonal anti-CD20 antibody
11th ÖGMBT Annual Meeting, Salzburg (Austria), 16.–18.09.2019

Müller K.
A novel approach for the comparison of selected monoclonal antidboy therapeutics by CZE-ESI-MS
17th Austrian Proteomics and Metabolomics Research Symposium (APMRS 2019), Salzburg (Austria), 18.–20.09.2019

Müller K.
A novel approach for the comparison of selected monoclonal antidboy therapeutics by CZE-ESI-MS
15th ASAC JunganalytikerInnen Forum 2019, Linz (Austria), 23.–26.09.2019

Wohlschlager T.
More than amino acids: protein modifications under scrutiny
Österreichische Chemietage 2019, Linz (Austria), 24.–27.09.2019

Schubert M.
The NMR signature of gluconoylation - a frequent N-terminal modification of recombinant proteins
Österreichische Chemietage 2019, Linz (Austria), 24.–27.09.2019

Hinterholzer A.
Detection and quantification of post translational modifications in biopharmaceuticals with NMR spectroscopy
Österreichische Chemietage 2019, Linz (Austria), 24.–27.09.2019

Poster Presentations

Hinterholzer A.
Glycan analysis of biotherapeutics using NMR spectroscopy in biopharmaceuticals
GlycoBioTec 2019, Berlin (Germany), 28.–30.01.2019 

Schubert M.
Gluconoylation - a frequent N-terminal modification - studied in isotope-labeled proteins by NMR spectroscopy
GlycoBioTec 2019, Berlin (Germany), 28.–30.01.2019 

Wohlschlager T.
Native mass spectrometry unravels highly complex glycosylation patterns in biopharmaceuticals
1st European Top-Down Proteomics Symposium, Paris (France), 12.–14.02.2019

Müller K.
Characterization of monoclonal antibody therapeutics by capillary electrophoresis electrospray-ionization mass spectrometry
HPLC 2019, Milano (Italy), 16.–20.06.2019

Schubert M.
Applying NMR spectroscopy to monitor modifications and glycosylation in protein biotherapeutics in biopharmaceuticals
EUROISMAR 2019 (combined EUROMAR 2019, ISMAR 2019, and the 41st GDCh FGMR annual meeting), Berlin (Germany), 28.–30.01.2019

Di Marco F.
Assessinig the higher order structure of human Chorionic Gonadotropin (hCG) by mass spectrometry-based approaches
17th Austrian Proteomics and Metabolomics Research Symposium (APMRS 2019), Salzburg (Austria), 18.–20.09.2019

Regl C.
A Tough Nut to Crack: Characterization of the Glycosylated Natural Allergen Art v 1 by Mass Spectrometry and NMR
17th Austrian Proteomics and Metabolomics Research Symposium (APMRS 2019), Salzburg (Austria), 18.–20.09.2019

Böttinger K.
Monitoring multiple attributes of biopharmaceutical proteins in bioprocess optimization
17th Austrian Proteomics and Metabolomics Research Symposium (APMRS 2019), Salzburg (Austria), 18.–20.09.2019

Hinterholzer A.
Detection and quantification of posttramslational modifications in biopharmaceuticals using NMR spectroscopy
17th Austrian Proteomics and Metabolomics Research Symposium (APMRS 2019), Salzburg (Austria), 18.–20.09.2019

Müller K.
A novel approach for the comparison of selected monoclonal antidboy therapeutics by CZE-ESI-MS
17th Austrian Proteomics and Metabolomics Research Symposium (APMRS 2019), Salzburg (Austria), 18.–20.09.2019

Hoppe I. J.
Analytical Cascades of Enzymes for Sensitive Detection of Structural Variations in Protein Samples
17th Austrian Proteomics and Metabolomics Research Symposium (APMRS 2019), Salzburg (Austria), 18.–20.09.2019

Kohlberger M.
Specific recognition of the heat induced conformation of the anti-CD20 antibody rituximab by single-stranded oligonucleotides known as aptamers
17th Austrian Proteomics and Metabolomics Research Symposium (APMRS 2019), Salzburg (Austria), 18.–20.09.2019

Brandstetter H.
ExteNDing proteome coverage with legumain as highly specific digestion protease
17th Austrian Proteomics and Metabolomics Research Symposium (APMRS 2019), Salzburg (Austria), 18.–20.09.2019

Müller K.
Middle down analysis of a therapeutci antibody with CZW-MS applying an in-lab designed interface
Österreichische Chemietage 2019, Linz (Austria), 24.–27.09.2019

Hinterholzer A.
Detection and quantification of posttramslational modifications in biopharmaceuticals using NMR spectroscopy
9th Austrian Peptide Symposium, University of Vienna, Vienna (Austria), 05.12.2019

Participation

Wohlschlager T.
MassSpec-Forum 2019, Vienna (Austria), 19.–20.02.2019

Schubert M.
Scientific Symposium in Honor of Prof. em. Dr. Beat Ernst, "Finding the Sweet Spot on the Journey towards Carbohydrate-Based Drugs", Basel (Switzerland), 12.04.2019

Stutz H.
15. ASAC JunganalytikerInnen Forum 2019 (JAF 2019), Linz (Austria), 23.–24.09.2019

Stutz H.
18th Austrian Chemistry Days, Linz (Austria), 24.–27.09.2019

Scientific Awards and Honours

Wohlschlager T.
Feigl-Price
Austrian Society for Analytical Chemistry, 25.9.2019

Wildner S.
Publication of the Month February
Department of Biosciences, University of Salzburg, for her paper in Scientific Reports (see above)

Hinterholzer A.
Publication of the Month October
Department of Biosciences, University of Salzburg, for his paper in Analytical Chemistry (see above)

Wildner S.
Publication of the Month November
Department of Biosciences, University of Salzburg, for her paper in Molecular Immunology (see above)

Müller K.
Poster Award
Austrian Proteomics and Metabolomics Research Symposium 2019

Organisation of Scientific Events

36th Winterschool on Proteinases and Inhibitors
Tiers (Italy), 13.–17.03.2019 

17th Austrian Proteomics and Metabolomics Research Symposium (APMRS) 2019
Salzburg (Austria), 18.–20.09.2019 

National and International Cooperations

C. Herwig
CD-Laboratory for Mechanistic and Physiological Methods for more Efficient Bioprocesses, Technical University Vienna, Austria

A. Bathke, W. Trutschnig
Statistics and Probability Research Group, Department of Mathematics, University of Salzburg, Austria

H. Lindner
Institute of Clinical Biochemistry, Biocenter, Medical University of Innsbruck, Austria

N. Kelleher
Northwestern University, Chicago, IL (USA)

S. Cianferani
Université de Strasbourg, Strasbourg (France)

J. Bones
National Institute for Bioprocessing Research & Training (NIBRT), Dublin (Ireland)

O. Kohlbacher
Applied Bioinformatics Group, University of Tübingen, Tübingen (Germany)

S. Koen
Research Institute for Chromatography, Katrijk (Belgium)

S. Li
University of California, San Diego, CA (USA)